HubLE Methods

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Novel and standard laboratory protocols presented in a ‘recipe’ style providing step-by-step descriptions of procedures in musculoskeletal research.

Quality control (QC) protocol for Genome Wide Association Study (GWAS) data

Sachin Wani & Nerea Alonso

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Morphometric parameters in bone analysis by microCT: which to use?

Phil Salmon

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Culture of the IDG-SW3 osteocyte cell line

Bethan K Davies & Isabel R Orriss​

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In vivo micro-computed tomography analysis of the mouse tibia

Enrico Dall’Ara  & Bryant Roberts​

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Culture of MLO-A5 and MLO-Y4 osteocyte cell lines

Luke Tattersall & Karan M. Shah

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Isolation and culture of primary rodent osteoblasts

Lucie E Bourne &​ Isabel R Orriss

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Isolation, generation and culture of bone marrow derived mouse osteoclasts

Silvia Marino & Mattia Capulli​

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Assessment of muscle mass and strength in mice

Andrea Bonetto, Daniel C Andersson
and David L Waning

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Ex vivo bone-myeloma organ cultures

Adam Ferrari & Jesus Delgado-Calle

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TRAcP
Staining

Aymen I Idris & Robert van’t Hof

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Generation of murine
adipocytes

Boya Li & Aymen Idris

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